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. 2018 Aug 6;8:11777. doi: 10.1038/s41598-018-30122-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Real-time PCR validation of top up or downregulated TFs and ERs in ESC and HC11 MEC undergoing lactogenic differentiation. (A) Bar chart showing real-time PCR measured relative expression of upregulated and (B) downregulated TFs along with its respective RNA-seq FPKM values (A’ and B’) in ESC, N, P and PRL treated HC11 cells. (C) Bar chart showing real-time PCR measured relative expression of up-regulated and (D) down-regulated ERs along with its respective RNA-seq FPKM values (C’ and D’) in ESC, N, P and PRL treated HC11 cells.