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. 2018 Aug 6;8:11754. doi: 10.1038/s41598-018-30293-0

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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High-density sub-volumes in the 3D EM reconstruction. (A) ResMap⁵⁹ was used to compute local resolution. (i) 2D slices taken at three positions (1,2,3) in the 3D-reconstruction with color-coded estimated local resolution. (ii) Local resolution maps of the three slices. Distance in nanometers. (iii) Vertical bar shows color scale for the local resolution (in angstroms). (B) (i) Density distribution of the rotationally averaged reconstruction⁷⁷. Vertical bars show electron density thresholds used for the half-maps (blue = 0.475, green = 0.45, gold = 0.385). (ii) Changes in the rotationally-averaged map as threshold is decreased from 0.475 (volume = 0.76*(10^6) cubic angstroms) to 0.45 (volume = 2.14*(10^6) cubic angstroms) to 0.385 (volume = 5.93*(10^6) cubic angstroms).