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. 2018 Aug 6;8:11750. doi: 10.1038/s41598-018-30289-w

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Constitutive and LL-37/hBD-2-induced NOD1, NOD2, and RIG-I mRNA and protein expression in PMCs. PMCs were incubated with medium alone (native, non-stimulated cells; NS), LL-37 or hBD-2 at a final concentration of 1 µg/mL. Receptor expression was assessed by (a) qRT-PCR and (b) WB. ACTB was used as control. Data represents the mean ± SD of three independent experiments performed with duplicate samples (each experiment was performed on 4 animals/PMC isolates). Differences were considered significant at P < 0.05 and are labeled with an asterisk (*) on each graph (Student’s t-test). One representative blot of three independent experiments performed with duplicate samples is shown. The cropped blots are used in the figure, and the full-length blots are presented in Supplementary Fig. S1.