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Primer sequences used for real-time PCR for human samples
| Gene | Sequence (sense, antisense: 5′–3′) |
|---|---|
| MAP1LC3B | AGCAGCATCCAACCAAAATC |
| CCGTTCACCAACAGGAAGAA | |
| BECN1 | TCCACAGAAAGTGCCAACAG |
| GTCAAAAAGGTCCCCAGTGA | |
| ATG5 | GCCATCAATCGGAAACTCAT |
| CAGCCACAGGACGAAACAG | |
| SQSTM1(p62) | TAGGAACCCGCTACAAGTGC |
| GAGAAGCCCTCAGACAGGTG | |
| RANKL | GATGAAAGGAGGAAGCACCA |
| TAAGGAGGGGTTGGAGACCT | |
| RANK | GCCTTTCTCTTGGCATCATC |
| TGCTTTTCCCCAGTATTTGC | |
| OPG | CAGGCACTTGAGGCTTTCA |
| TTGGGGTTTATTGGAGGAGA |
