Fig. 5.

Inhibition of miR-221/222 decreases migration and invasion ability in MDA-MB-231 breast cancer cells. a, b Representative panels from the scratch wound assay in MDA-MB-231 cells imaged by microscopy. MDA-MB-231 cells were transfected with a miR-221/222 inhibitor or miR-control. Wound healing was measured in 5 random fields. Data represent the mean of triplicate experiments ± SEM. c, d Representative micrographs (×200) of matrigel-coated or non-coated Transwell assays in MDA-MB-231 cells transfected with miR-221/222 inhibitors or miR-NC. Cells were photographed to measure wound length at 0 and 24 h. Invading or migrating cells were counted in five random fields. Data represent the mean of triplicate experiments ± SEM. Scale bars represent 50 μm. *P < 0.05; **P < 0.01; ***P < 0.001