Table 1.
Reactions and kinetic parameters of B. subtilis used for modelling.
| Enzyme | Enzyme (full name) | EC | Reaction | KM (mM) | Reference |
|---|---|---|---|---|---|
| Transport | |||||
| PTSg | phosphotransferase system | 2.7.1.9 | glucose + PEP → G6P + pyruvate | 20 | 43 |
| Irreversible reaction | |||||
| G6PDH | glucose 6-phosphate dehydrogenase | 1.1.1.49 | G6P + NADP ↔ 6PG + NADPH + H | 0.075 | 44 |
| Pfk | 6-phosphofructokinase | 2.7.1.11 | F6P + ATP ↔ FBP + ADP + H | 0.12 | this study |
| Fbp | fructose-1,6-biphosphatase | 3.1.3.11 | FBP + H2O ↔ F6P + Pi | 0.1 | 35 |
| GapA | glyceraldehyde-3-phosphate dehydrogenase | 1.2.1.12 | GAP + NAD + Pi ↔ BPG + NADH + H | 0.1 | 21 |
| GapB | glyceraldehyde-3-phosphate dehydrogenase | 1.2.1.13 | BPG + NADPH + H ↔ GAP + NADP + Pi | 0.86 | 21 |
| Pyk | pyruvate kinase | 2.7.1.40 | PEP + ADP + H ↔ pyruvate + ATP | 0.25 | 45 |
| PycA | pyruvate carboxylase | 6.4.1.1 | pyruvate + ATP + CO2 ↔ OAA + ADP + Pi + H | 0.10 | this study |
| Pdh | pyruvate dehydrogenase | 1.2.4.1 | pyruvate + NAD ↔ acetyl-CoA + NADH + CO2 | 0.4 | 46 |
| PckA | phosphoenolpyruvate carboxykinase | 4.1.1.49 | OAA + ATP ↔ PEP + ADP + CO2 | 0.025 | 22 |
| Reversible reaction | |||||
| Pgi | glucose 6-phosphate isomerase | 5.3.1.9 | G6P ↔ F6P | — | |
| Fba | fructose-1,6-biphosphate aldolase | 4.1.2.13 | FBP ↔ DHAP + GAP | — | |
| Tpi | triose phosphate isomerase | 5.3.1.1 | DHAP ↔ GAP | — | |
| Fba + Tpi ( = Ald) | FBP ↔ 2 DHAP | — | |||
| Pgk | phosphoglycerate kinase | 2.7.2.3 | BPG + ADP ↔ 3PG + ATP | — | |
| Pgm | phosphoglycerate mutase | 5.4.2.12 | 3PG ↔ 2PG | — | |
| Eno | enolase | 4.2.1.11 | 2PG ↔ PEP + H2O | — | |
| Pgk + Pgm + Eno ( = Eno) | xPG ↔ PEP | — | |||