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. 2018 Aug 6;4:86. doi: 10.1038/s41420-018-0088-8

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 1 — a, b The total KLF12 protein levels were normalized to GAPDH expression, and the data for all of the endometrial samples are shown in the scatter plots. ^***P < 0.001 compared with the FER group. c, d Immunohistochemical analysis was performed using KLF12 antibody. Endometrial tissue samples from fertile women and RIF patients are shown at ×200 (left panel) and ×400 (right panel) magnification. The negative control (NC) is nonspecific rabbit serum. Red represents positive staining (arrows). Scale bars, 100 μm (left panel) and 50 μm (right panel). The means and integrated optical densities of KLF12 expression in the endometrial tissue were calculated