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. 2018 Aug 6;4:86. doi: 10.1038/s41420-018-0088-8

Fig. 5. KLF12 directly repressed LIF transcription in Ishikawa cells.

Fig. 5

a Four LIF-Luc reporter constructs were designed according to the schematic diagram of partial LIF promoter sequence. These constructs were named as LIF-Luc1/2 and LIF-Luc Mut1/2. b Ishikawa cells were infected with the indicated adenoviruses for 24 h and then transfected with LIF-Luc1/2 or LIF-Luc MUT1/2. After 48 h, luciferase assays were performed, and the data were plotted after normalization to Renilla luciferase activity. ***P < 0.001 and ###P < 0.001 compared with Ad-LacZ alone. c ChIP-PCR amplification using primers against the human LIF promoter region. PCR products were separated by agarose gel electrophoresis. Input (non-precipitated) chromatin was utilized as a positive control in these analyses. d ABCD assays were performed using biotinylated or non-biotinylated (competitor) double-stranded LIF wild-type (WT) and conserved element-mutated (MUT) oligonucleotides with whole-cell extracts from Ishikawa cells