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. 2018 Aug 6;8:11756. doi: 10.1038/s41598-018-30187-1

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Effect of the length of r-DNA1 and r-DNA2 conjugated flexible MTs on the mode of swarming of the MTs. (a) Histograms of the length of r-DNA1 conjugated MTs (red MTs) and r-DNA2 conjugated MTs (green MTs) just after their preparation. The fluorescence microscopy image shows the swarms of MTs which exhibited circular motion. The average lengths of the red and green flexible MTs were 22.2 ± 12.5 µm and 16.2 ± 9.0 µm respectively. (b) Histograms of the length of the r-DNA1 conjugated MTs (red MTs) and the r-DNA2 conjugated MTs (green MTs) after shear treatment (ten times). The fluorescence microscopy image shows the swarms of MTs which exhibited translational motion. The length of the red and green flexible MTs was 4.7 ± 2.5 µm and 4.7 ± 2.4 µm respectively. The number of MTs considered for the length measurement was 50 in each case. The images were captured after 60 min of the addition of l-DNA and ATP. The concentration of the red and green MTs was 0.6 μM each. The concentration of l-DNA was 0.6 μM. The concentration of kinesin was 0.3 μM. Scale bar: 50 µm.