Fig. 2.

TRPS1 represses YAP target genes. a Western blot for YAP from MCF7 i5SA (pInducer-Strep YAP 5SA MCF7) cells after 14-h induction by doxycycline (DOX) or ethanol (EtOH), respectively. Vinculin serves as loading control. b MA plot for RNA-Sequencing from MCF7 i5SA cells after doxycycline (DOX) or ethanol (EtOH) treatment for 14 h. The Log₂ fold change (Log₂FC) is plotted against the abundance of transcripts given as number of reads per kilobase per million mapped reads (RPKM). YAP-induced genes are drawn as blue dots. c Western blot for TRPS1 and YAP after infection of MCF7 cells with three different TRPS1 shRNAs (shTRPS1 #1–3) or a non-targeting control (shNTC). Vinculin serves as loading control. d Gene set enrichment analysis (GSEA) for a set of 497 YAP-induced genes in RNA-Sequencing from TRPS1-depleted MCF7 cells. NES normalized enrichment score. e MA plot for RNA-Sequencing from MCF7 cells infected with three different TRPS1 shRNAs or a non-targeting control (shNTC). The Log₂ fold change (Log₂FC) is plotted against the abundance of transcripts given as number of reads per kilobase per million mapped reads (RPKM). YAP-induced genes are drawn as blue dots. Red dots label established YAP targets. f Violin Plot showing the differential regulation of YAP-induced genes after TRPS1 depletion by shTRPS1.The white point indicates the median. Two-sided Wilcox test. g qRT-PCR analysis of several YAP target genes in MCF7 i5SA cells after induction of YAP 5SA expression by doxycycline (DOX) or ethanol control (EtOH). Data presented are means from three biological replicates (n = 3) and error bars represent s.e.m; *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t-test. h qRT-PCR analysis of the YAP target genes from g in MCF7 cells infected with three different TRPS1 shRNAs or a non-targeting control (shNTC). Data presented are means from technical triplicates per shRNA and error bars represent s.d.; *P < 0.05; **P < 0.01; ***P < 0.001; Student’s t-test