Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2018 Aug 6;9:3115. doi: 10.1038/s41467-018-05370-7

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

PMC Copyright notice

Fig. 8 — TRPS1 is needed for efficient tumour growth in vivo. a Western blot for TRPS1 in 4T1 cells infected with two different shRNAs targeting TRPS1 or a non-targeting shRen (Renilla) control, respectively. Serial dilutions (lanes 1–3) of the shRen control are loaded to demonstrate the knockdown efficiency. b Tumour growth of 4T1-derived tumours orthotopically transplanted into the mammary gland of BALB/C mice. Tumour size was monitored after the indicated times of injection. Data are presented as means ± s.e.m. Two-way ANOVA test. c The tumour weight of the 4T1-derived tumours was determined 23 days after transplantation. One-way ANOVA test. d Photos of 4T1-derived tumours 23 days after transplantation. Scale bar = 1 cm. e Representative images of IHC stainings for CD3-positive cells (T cells) in sections of the 4T1-derived tumours. Scale bar = 50 μm. f Quantification of (k). Error bars represent s.e.m. One-way ANOVA test. g Expression of signature gene sets for natural killers (NK), cytotoxic T cells (CD8) and helper T cells (CD4) were sorted based on TRPS1 activity in breast cancer patients. Spearman rank correlation test