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. 2018 Aug 6;8:11766. doi: 10.1038/s41598-018-29580-7

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Flow cytometry analysis of P. acnes P0 strain growing in biofilms and exposed to several concentrations of AS-48 and AS-48 plus lysozyme, stained with acridine orange and propidium iodide⁴¹. Percentage of dead cells after the tratments, according to the flow cytometry analysis in relation to control of death (80 °C, 20 min). *Significant differences according to Anova one-way Posthoc LSD (p < 0.05).