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. 2018 Aug 6;8:11739. doi: 10.1038/s41598-018-30168-4

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© The Author(s) 2018

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Immunohistochemical (IHC) Analysis of NANOG Expression in Different Renal Cell Carcinoma (RCC) Samples. RCC samples expressed NANOG at various levels. Nuclear expression of NANOG in clear cell RCC at various levels is as follows: weak (a), moderate (b), and strong (c); chromophobe RCC: weak (d), and moderate (e); and papillary RCC: weak (f), and strong (g). Cytoplasmic expression of NANOG in clear cell RCC at various levels is as follows: weak (h), moderate (i), and strong (j); chromophobe RCC: moderate (k), and strong (l); papillary RCC: moderate (m), and strong (n). IHC staining of seminoma tissue is presented as negative (o) and positive (p) controls. Figures are demonstrated with a magnification of 200x.