Figure 1.

Tamoxifen treatment did not affect I/R-incited renal injury at day 5. A. Scheme of the experimental. B. Kidney gross appearance in each group. C. Statistical analysis of the ratio (left/right) of kidney weight. D. Scr and BUN levels among different groups. E. PAS staining. F. Kim-1 represented injured tubules. Semi-quantitative analysis of immunofluorescence staining of Kim-1 in the kidney, based on the positive area of Kim-1 in 10~15 random areas for all mice collected. Original magnification × 400. Scale bar =50 µm. G. Renal injury biomarkers KIM-1 and NGAL were detected by RT-PCR. Statistical differences between groups were analyzed by one-way ANOVA followed by Tukey’s post-tests. Values are mean ± SEM. (N=4/group. *P<0.05, **P<0.01, ***P<0.001).