Figure 2.

Tamoxifen treatment did not modify tubular injury and apoptosis in kidneys after I/R at day 5. (A) LTL: lotus tetraglonolobus lectin labeled normal proximal tubule. (B) DBA: dolichos biflorusagglutinin represented normal collecting duct. Original magnification × 100 (C) Apoptosis cells were stained by TUNEL. LTL (D), DBA (E) and TUNEL + cells (F) was calculated in 15 visual fields. (G) Expression of Caspase 3, Bax, and Bcl-2 were detected by western blot. Quantitation of Caspase 3, Bax, and Bcl-2 was normalized by β-actin. Statistical differences between groups were analyzed by one-way ANOVA followed by Tukey’s post-tests. Scale bar =50 µm. The data shown are mean ± SEM. (N=4/group. *P<0.05, **P<0.01, ***P<0.001).