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Expression of total VEGFR-1 in cells at 24 (A) and 48 (B) hours post transfection. Effect of selective SiRNA inhibition of VEGF isoforms, VEGF receptors, and/or IGF-I on sVEGFR-1 levels in the media of proliferating human retinal endothelial cells (HRECs) at 24 (C) and 48 (D) hours post transfection. Data are expressed as mean ± SEM (n=12 samples/group; *P<0.05, ***P<0.01 vs. control). Images were captured at 20× magnification.
