Table 1.
Gene expression of angiogenic growth factors in human retinal endothelial cells in response to 24-hour SiRNA exposure
| Genes of Interest | Total VEGF | VEGF189 | VEGF165 | VEGF121 | VEGFR-1 | VEGFR-2 | NP-1 | NP-2 | IGF-I | IGF-2 | IGF-IR | IGF-2R | Total VEGF+IGF-I | VEGF189+IGF-I | VEGF165+IGF-I | VEGF121+IGF-I |
|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
| Casp 9 | 2.4 | 3.2 | 8.8 | 1.6 | 1.8 | 1.3 | 3.5 | 1.7 | -2.0 | 2.2 | 1.2 | 7.4 | -1.1 | 17.5* | 76.4 | 74.5* |
| Cav 1 | 1.0 | -1.2 | -1.3 | -1.1 | -1.4 | -1.6 | -2.2 | -1.2 | -1.5 | -1.2 | -1.7 | -2.1 | -1.8 | -1.4 | -1.1 | -1.6 |
| COX-2 | 1.2 | 1.0 | -2.0 | 1.4 | 1.5 | 1.6 | -1.3 | 2.0 | -4.7 | 1.5 | 2.5 | 1.6 | 1.8 | 3.8 | 16.5* | 16.1* |
| eNOS | -1.0 | 1.1 | 1.1 | 2.4 | -3.0 | 1.2 | 1.2 | -1.3 | 1.2 | 1.3 | 2.3 | -1.0 | 1.0 | 1.6 | 6.9 | 6.7 |
| HIF1α | -1.1 | 1.1 | 1.1 | 1.3 | 1.2 | -1.3 | 1.1 | 1.2 | -1.2 | -1.0 | 1.2 | -2.5 | -1.1 | -1.7 | 2.6 | 2.5 |
| MAPK 1 | -1.0 | 1.1 | 1.2 | 1.6 | -2.0 | -1.1 | -1.0 | -1.2 | -1.2 | 1.0 | -1.1 | -1.7 | -1.1 | -1.2 | 2.8 | 2.7 |
| MAPK 3 | 1.1 | 2.1 | 21.3* | 4.2 | 11.1 | 2.5 | 26.0* | 13.3* | 5.3 | 2.5 | 18.3* | 74.5* | 13.5* | 122.4* | 2553* | 553.7* |
| NP-1 | 1.1 | 1.4 | 2.1 | 1.7 | 1.4 | 1.2 | -3.0 | 1.2 | -1.4 | 1.1 | 1.4 | 4.1 | 1.2 | 4.6 | 69.7 | 45.6* |
| NP-2 | 1.5 | 1.2 | 1.2 | 1.5 | -1.0 | -1.0 | 1.2 | -1.3 | 1.2 | -1.1 | 1.7 | -4.3 | 1.3 | -3.1 | 1.4 | 1.4 |
| PDGF | 1.2 | 2.2 | 5.0 | 3.2 | -1.3 | -1.3 | 1.6 | -1.3 | 1.0 | -1.2 | 1.3 | 3.3 | 1.9 | 7.8 | 44.6 | 33.1* |
| PKCα | -1.1 | 1.6 | -1.0 | 2.0 | 2.0 | -1.1 | 2.0 | 1.5 | 1.22 | -1.0 | 1.2 | 2.1 | 1.1 | 3.4 | 14.9 | 14.5* |
| PKCβ | 1.4 | 3.0 | 35.2* | 4.9 | 12.8* | 5.2 | 41.8* | 11.7* | 20.3* | 4.2 | 23.8* | 108.7* | 37.8* | 299.9* | 1691* | 3852* |
| PKCγ | -1.4 | 1.8 | 28.6* | 4.8 | 19.4* | 4.1 | 38.5* | 18.3* | 5.4 | -1.1 | 13.5* | 81.2* | 9.8 | 191.3* | 835.5* | 814.2* |
| VEGF A | 1.2 | 1.3 | -1.4 | -1.1 | 1.6 | 1.3 | 1.8 | 2.6 | -3.9 | -1.0 | 2.0 | 4.0 | -2.2 | 9.1 | 39.8* | 38.8* |
| VEGF B | -1.0 | 1.1 | -1.5 | 1.2 | 1.4 | 1.2 | -1.2 | -3.0 | 1.5 | 1.2 | 1.2 | -2.8 | -3.0 | -1.2 | 3.6 | 3.5 |
| VEGF C | -1.2 | -1.6 | 1.4 | 1.5 | 1.2 | 1.0 | 1.9 | -1.3 | -1.5 | 1.2 | 1.7 | 5.0 | 1.8 | 7.2 | 31.6* | 30.8* |
| VEGF D | -1.3 | 1.5 | 19.9* | 2.6 | 9.2 | 2.4 | 23.0 | 11.0* | 3.0 | 1.1 | 7.5 | 36.8* | 7.1 | 110.4* | 311.4* | 401.4* |
| VEGFR-1 | 1.2 | 1.0 | -3.0 | 1.1 | -4.3 | -2.2 | -1.0 | -1.5 | -1.3 | -1.6 | -1.2 | -1.0 | -8.5 | 2.3 | 10.0* | 9.8 |
| VEGFR-2 | 1.0 | 1.0 | 1.7 | 1.3 | -1.3 | -2.0 | 1.0 | 1.1 | -1.1 | -1.8 | 1.0 | 3.0 | -2.2 | 3.3 | 8.8 | 7.2 |
| VEGFR-3 | 1.1 | 1.7 | 1.2 | 1.7 | -1.5 | -2.3 | 1.5 | 4.7 | 1.5 | 1.0 | 2.7 | 2.8 | -1.6 | 6.6 | 188.2* | 27.9* |
Data are fold change from control (no knockdown). All data were corrected using 5 different housekeeping genes. Genes are selected from a profile of 84 genes. Genes of interest are: Casp 9, caspase 9; Cav 1, caveolin-1; COX-2, cyclooxygenase-2; eNOS, endothelial cell nitric oxide synthase; HIF1a, hypoxia inducible factor 1a; MAPK, mitogen-activated protein kinase; NP, neuropilin; PDGF, platelet-derived growth factor; PKC, protein kinase C; VEGF, vascular endothelial growth factor; VEGFR, VEGF receptor.
*
p<0.05 versus control.