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. 2018 Jul 15;10(7):1976–1989.

Figure 6.

Figure 6

HDAC4 is required for Ang II-mediated α-SMA expression and ERK1/2 activation. (A) SiRNA or negative control (NC) siRNA targeting HDAC4 were transiently transfected into pericytes. The pericytes were subsequently cultivated in the presence or absence of Ang II for 12 h. Quantitative PCR (Q-PCR) was performed to examine HDAC4 (A) and α-SMA (B) mRNA levels (n = 3, means ± SEM). *P < 0.05 vs untreated control; #P < 0.05 vs Ang II alone treated group. (C) HDAC4 knockdown and control pericytes were cultivated in the presence or absence of Ang II for 24 h. Western blot analysis was performed using α-SMA and β-actin antibodies, and then the membranes were stripped and probed for pERK1/2 and total-ERK1/2 (tERK1/2) (D).