HER2 expression in GC cell lines. A. Detection of HER2 and β-tubulin by western blot analysis of cells from three metastatic GC cell lines (HGC-27, SGC-7901, NCI-N87) and two primary GC cell lines (BGC-823, AGS). B. HER2 bands were normalized to β-tubulin. The data are expressed as the means ± SD from three independent experiments. C. Immunofluorescence imaging of HER2 (red), LDHA (green), and nucleus labeled as DAPI (blue), and the co-localization of the three signals (merge) in SGC-7901, HGC-27, and NCI-N87.