Figure 3.

Silencing of LDHA inhibits cell invasion and migration. SGC-7901, HGC-27, and NCI-N87 cells were transfected with LDHA siRNA for 48 h (A, E, I). The expression of LDHA in the three cell lines was detected by western blot analysis (B, F). The wound healing assay was carried out at 0 and 24 h to observe the migration ability of the HGC-27 and SGC-7901 after down-regulation of LDHA (C, G, J). Transwell migration and invasion experiments with SGC-7901, HGC-27, and NCI-N87 were performed and microscopic images (200× magnification) were acquired (D, H, K). Differences of the cell migration and invasion between the RNA interference group and the control group are shown. Each group of cells was counted by five fields of view. The data are expressed as the means ± SD from three independent experiments. The data were analyzed by a two-tailed Student’s t test. ***: P < 0.001.