Figure 4.

RSV promotes cell viability and protects LPS-induced apoptosis in MH-S cells. RSV decreased LPS-induced apoptosis in MH-S cells. A, B. Viability of MH-S cells was estimated using the MTT assay. Results are representative of three independent experiments, mean ± SD, *P<0.05 compared with control. C. Apoptosis of MH-S cells were stained with Annexin-VFITC and PI and analyzed by flow cytometry. D. Quantitative analysis of cell apoptosis in MH-S cells. E. Representative western blots show LPS-induced expression of Bax and Bcl-2 in MH-S cells with and without pre-treatment with RSV. F. Densitometric analysis shows that treatment with LPS reduced the Bcl-2/Bax ratio, and that this effect could be inhibited by RSV pre-treatment. *P<0.05 compared with control; #P<0.05 compared with LPS treated alone.