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. 2018 Jul 15;10(7):1961–1975.

Figure 6.

Figure 6

RSV protects AMs against LPS-induced apoptosis in part via inhibiting LPS-induced autophagy. RSV decreased LPS-induced autophagy in MH-S cells. (A) Autophagy level in MH-S cells was detected by MDC staining (B) RSV decreased protein level of LC3-II/I in MH-S cells (C) LC3-II/I protein levels after siRNA treatment in MH-S cells. (D) Cell apoptosis was detected using Annexin V/PI staining and analyzed by flow cytometry. (E) Quantitative analysis of cell apoptosis in MH-S cells. Quantitative analysis of cell apoptosis in MH-S cells, *P<0.01 compared with control. #P<0.05 compared with LPS treated alone &P<0.05 compared with the RSV+LPS group. (F) LC3-II/I expression was detected using western blot after rapamycin treatment in MH-S cells.