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Experimental protocol for creating an alpha-naphthylisothiocyanate (ANIT)-induced sclerosing cholangitis model. Rats received 100 mg/kg ANIT twice weekly for 4 weeks. One million human amnion-derived mesenchymal stem cells (hAMSCs) or 200 μL of conditioned medium (CM) were intravenously administered on days 15 and 22. All rats were sacrificed on day 29.
