Figure 2.

Dysregulation LINC00673 gene expression in PTC cells inhibiting proliferation and metastasis. A, C. MCF-7 and MDA-MB-231 cell lines transfected with Si-RNA or Si-NC were cultured in 96-well plates for 1-4 days and using CCK-8 measured cell proliferation. Cell proliferation was significantly suppressed in breast cancer cell transfected by Si-RNA. E, G. MDA-MB-231 and BT-549 cell lines transfected with LINC00673 or Vector were cultured in 96-well plates for 1-4 days and using CCK-8 measured cell proliferation. Cell proliferation was significantly enhanced in breast cancer cell transfected by Si-RNA. B, D. MCF-7 and MDA-MB-231 cell lines transfected with Si-RNA or Si-NC were cultured in 6-well plates for 10-14 days. F, H. MDA-MB-231 and BT-549 cell lines transfected with LINC00673 or Vector were cultured in 6-well plates for 10-14 days. I, J. In MCF-7 and MDA-MB-231, transwell migration assays in down-regulation LINC00673 cells and their corresponding control cells. K, L. In MDA-MB-231 and BT-549, transwell migration assays in up-regulation LINC00673 cells and their corresponding control cells. M, N. In MCF-7 and MDA-MB-231, transwell invasion assays in down-regulation LINC00673 cells and their corresponding control cells. O, P. In MDA-MB-231 and BT-549, transwell invasion assays in up-regulation LINC00673 cells and their corresponding control cells. The columns represented the mean number from at least three independent experiments, and the little vertical bars on the top of the columns represent SD. *P < 0.05, **P < 0.01 and ***P < 0.001 in comparison with Si-NC or si-LINC00673 and LINC00673 or Vector using student’s t-test.