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. 2018 Jul 1;8(7):1228–1238.

Figure 4.

Figure 4

Functional relationship between miR-1292-5p and the 3’UTR of DEK. A. Luciferase reporter plasmids were constructed by the insertion of the full-length NRP1 3’UTR into a region immediately downstream of the luciferase gene. B. The relative luciferase activity of the wild-type (3’WT UTR) and mutated DEK 3’UTR (3’ mutant UTR) in AGS/SGC-7901 cells. C. qPCR examination of DEK in control miRNA-overexpressing SCG-7901 cells, miR-1292-5p-overexpressing SCG-7901 cells, control Antagomir SCG-7901 cells and Antagomir-1292-5p SCG-7901 cells. D. Western bolt analysis of DEK in control miRNA-overexpressing SCG-7901 cells, miR-1292-5p-overexpressing SCG-7901 cells, control Antagomir SCG-7901 cells and Antagomir-1292-5p SCG-7901 cells. E. Representative immunohistochemistry results of staining for DEK in non-tumor and tumor samples. F. Quantification of DEK expression in gastric carcinoma tissues (C) and adjacent normal mucosa (N). Error bars indicate SD and *indicates P<0.05.