Table 2.
Morphological and phylogenetic characterization of endophytic actinobacteria isolated from Thai pigmented rice
| Morphological characteristicsa | Phylogenetic characteristicsb | ||||||
|---|---|---|---|---|---|---|---|
| Morphological group(No. of isolate) | Colony color | Spore color | Spore number | Soluble pigment | Group representative (GenBank accession no.) | % Identityc |
Closest phylogenetic species (GenBank accession no.) |
|
Group I (34): HN7, 10, 12, 16–20, 27 (9) LPR1–3, 5, 7, 9, 11–19, 22, 24, 25, LPL1–7 (25) |
Reddish brown | White | Pair of spores | Not produced |
HN10 (MG687439) LPL6 (MF397903) LPL7 (MF397907) LPR5 (MF397906) LPR12 (MF397904) LPR25 (MF397908) |
99.17 99.72 99.31 99.17 99.79 99.24 |
Microbispora hainanensis 211020T (FJ261972) |
|
Group II (3): LPR4, 8, 10 |
Red | White | Spherical spore | Produced in red |
LPR4 (MG687442) LPR10 (MF397909) |
98.72 98.65 |
Sphaerisporangium siamense SR14.14T (HM043727) |
|
Group III (29): HN1–6, 8, 9, 11, 13–15, 21–26, 28–33 (24) LPR6, 20, 21, 23, 26 (5) |
Cream | Gray | Chain of >20 spores | Not produced or Produced in red |
HN2 (MG687436) HN3 (MG687437) HN6 (MG687438) HN22 (MG687440) HN25 (MF397913) HN33 (MG687441) LPR23 (MF397914) LPR26 (MG687443) |
99.71 99.65 99.72 99.65 99.65 99.65 99.72 99.65 |
Streptomyces diastaticus subsp. ardesiacus NRRL B‐1773T (DQ026631) |
a
Morphological characterization was carried out after growing endophytic actinobacteria on HT agar medium for 14 days.
b
The 16S rRNA gene sequences of the group representatives were deposited in GenBank and used for phylogenetic analysis (Figure 1).
c
The % identity refers to the percentage of the gene sequence similarity of the group representatives compared to their closest phylogenetic species.