Figure 2.

Feature analysis of purified Cry1B Nbs. (a) The specificity of purified Cry1B Nbs was tested by indirect ELISA with different types of Cry toxins. (b) The equilibrium dissociation constants between Cry1B and the paired Nbs (Nb2 and Nb3) was measured with the kinetic analysis by SPRi. Cry1B dilutions were injected at concentrations of 1, 3, 9, 27, 81, 243, and 729 nmol L^‐1 (C7–C1). (c) Relevant parameters of kinetic analysis. (d) The thermal stability of Nb2 and Nb3 was measured by a fluorescence‐based assay with Sypro Orange dye. The temperature was set from 25 to 98°C in increments of 1°C and three repeats were performed