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Pinene production by E. coli BW25113 (PT5-dxs, pMEVI) harboring mutant gene clusters from error-prone PCR (A) and by E. coli LYCOP harboring mutant gene clusters from DNA shuffling (B). Pinene concentrations were measured using the GC-FID (A) and the concentrated sulfuric acid (B) methods. The data represent the means of three replicates and error bars represent standard deviations.
