FIGURE 3.

Effects of ASP on TNF-α-induced activation of GATA2 and NF-κB and on inhibition of EPO induced by TNF-α in Hep3B cells under normoxia and hypoxia. (A) Expression of nuclear GATA2 in TNF-α-induced Hep3B cells. (B) Immunofluorescence analysis of NF-κB nuclear translocation by ASP treatment (200 μg/mL). Original magnification, 400×. (C) EPO mRNA expression in TNF-α-induced Hep3B cells. (D–F) Nuclear GATA2 and NF-κB expression of TNF-α-induced Hep3B cells cultured in the presence of CoCl₂ for 24 h. (G) EPO expression of TNF-α-induced Hep3B cells under hypoxia. One representative blot out of three independent experiments is shown, Histone H3.1 served as nuclear loading control. Values are means ± SEM from three independent experiments. ^##P < 0.01 compared with the control group; ^∗P < 0.05, ^∗∗P < 0.01 compared with the TNF-α group.