FIGURE 2.

Single-prolonged stress does not alter the amplitude of the hyperpolarization-activated current (I_h) in BLA neurons from either male or female rats. I_h was elicited from a holding potential of –40 mV by a series of hyperpolarizing voltage steps in 10 mV increments (2 s duration) to –110 mV. (A) Current-voltage relationships of I_h obtained from representative BLA neurons from male rats in the unstressed control (Control, ) and single prolonged stress (SPS, ) conditions. (B) Representative recordings of I_h elicited upon hyperpolarization to –110 mV in BLA neurons from male SPS or control male rats. (C) Activation curves of I_h in BLA neurons from Control male and female rats show no sex differences in voltage-dependence of the conductance underlying I_h. Current-voltage relationships and representative I_h recordings in BLA neurons from female Control and SPS rats are show in (D,E), respectively. Scale in (B) is the same in (E). (F) The amplitude of I_h evoked upon hyperpolarization to –110 mV in males (left; Control, N = 13, open bars; SPS, N = 7, filled bars) and females (right; Control, N = 12; SPS N = 6). (G) Shows a representative current elicited upon 2 s hyperpolarization from –40 to –110 mV. I_h is calculated as the difference between the instantaneous current (I_in) and the steady-state current (I_ss). Data are shown as mean ± SEM ^∗p < 0.05, compared to male/control condition (ANOVA followed by Student’s t-test. See text for details).