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. 2018 Jul 31;9:1779. doi: 10.3389/fmicb.2018.01779

FIGURE 1.

FIGURE 1

Tn5253 excises from pneumococcal chromosome producing a circular form and a reconstitution of attB insertion site. In the circular form of Tn5253 the left and right ends are joined by attTn which is identical to attR whereas the reconstituted attB site is identical to attL. att sites are represented as filled rectangles, chromosomal genes as open arrows, Tn5253 as a yellow bar. Arrowheads represent PCR primers used for circular form (dark gray) and reconstituted attB site (light gray) detection.