Table 2.

Oligonucleotide primers.

Name	Sequence (5′ to 3′)	Notesa	GenBank ID: nucleotides
IF327	CAA TAT AGC GTG ATG ATT GTA AT		EU351020: 1,103–1,081
IF328	AGT GAG AAT CAA ATC AGA GGT T		EU351020: 65,221–65,242
IF373	GAT GAT GAT TTG ACA CAA GAA TA		EU351020: 62,969–62,991
IF521	ATC AAA CGG ATC CCC AGC TTG TAT TCA TGT CAT CAT CCT TCC T	The first 21 nucleotides are complementary to IF149	EU351020: 63,439–63,418
IF522	ATA TTT TAC TGG ATG AAT TGT TTT AGT TTT GGT GTT CGC TTG GTG TTT AG	The first 26 nucleotides are complementary to IF210	EU351020: 65,260–65,283
IF523	CGG TGT ATC CAA GAT TTC CAG		EU351020: 65,862–65,842
IF517	ATT TCC TTG CGT GAT GTG TGA		EU351020: 16,810–16,830
IF681	GTA TCG CTC TTG AAG GGA ATA GTA CAA ATG AAT TTA CTA CTT	The first 19 nucleotides are complementary to IF101	EU351020: 17,305–17,283
IF682	GAT CCA CTA GTT CTA GAG CTC CCA AAT AGG AAT GTC AGT	The first 19 nucleotides are complementary to IF100	EU351020: 18,780–18,799
IF520	GTA TGG TCG TTG ATG AAG TCT		EU351020: 19,348–19,328
IF100	GCT CTA GAA CTA GTG GAT C		AY334020: 1–16
IF101	TTC CCT TCA AGA GCG ATA C		AY334020: 890–872
IF149	CAA GCT GGG GAT CCG TTT GAT		AY334018: 5–25
IF210	CTA AAA CAA TTC ATC CAG TAA AAT AT		AY334019: 880–855
IF496	GTT TGG ACA TCA TTC ATT TG		CP000410: 1,043,748–1,043,767
IF356	GAC TAG ATA GAG GCA AGC GT		CP000410: 1,043,962–1,043,943
IF138	CAG ATC AAG AAA TCA AAC TCC AA		CP000410: 725,024–725,046
IF139	CAG CAT CAT CTA CAG AAA CTC		CP000410: 725,194–725,174

^aNucleotides complementary to resistance cassettes primers are reported in italics.