Table 1.
Ciliate genera | Prokaryotic fraction* | Raw sequences | Quality sequences | No. of OTUs# | Chao1 | Simpson | Shannon | Phylogenetic diversity | Goods Coverage (%) |
---|---|---|---|---|---|---|---|---|---|
MONOCULTURES | |||||||||
Entodinium caudatum | FLP | 80,257a | 5,616a | 125a | 248a | 0.91b | 5.09 | 16.6a | 86.1b |
CAP | 14,882b | 1,182b | 78b | 96b | 0.95a | 5.24 | 12.3b | 95.9a | |
Epidinium caudatum | FLP | 66,454a | 6,505a | 102a | 230a | 0.85 | 4.35 | 15.4a | 88.1b |
CAP | 15,241b | 1,906b | 75b | 92b | 0.89 | 4.71 | 12.8b | 95.7a | |
FRESHLY ISOLATED CILIATES | |||||||||
Rumen fluid | FLP | 43,316 | 33,630 | 269a | 443a | 0.95 | 6.46a | 26.3 | 90.1b |
Dasytricha | CAP | 47,162 | 15,239 | 80b | 96b | 0.96 | 5.33b | 13.5c | 98.9a |
Diplodinium | CAP | 28,224 | 10,505 | 87b | 100b | 0.97 | 5.45b | 14.3bc | 98.8a |
Diploplastron | CAP | 46,066 | 24,845 | 130b | 144b | 0.97 | 6.02ab | 19.6b | 98.3a |
Entodinium | CAP | 39,364 | 20,029 | 111b | 128b | 0.96 | 5.56b | 16.2bc | 98.2a |
Epidinium | CAP | 32,375 | 16,601 | 95b | 110b | 0.97 | 5.66b | 15.4bc | 98.8a |
Isotricha | CAP | 34,608 | 12,699 | 97b | 111b | 0.96 | 5.56b | 15.5bc | 98.7a |
Ophryoscolex | CAP | 35,344 | 16,677 | 109b | 127b | 0.97 | 5.68b | 17.4bc | 98.3a |
Polyplastron | CAP | 44,884 | 21,163 | 99b | 116b | 0.94 | 5.39b | 15.7bc | 98.5a |
FLP, free-living prokaryotes; CAP, ciliate-associated prokaryotes. Means with different superscripts within columns between the FLP and the CAP of each monoculture or between the FLP and the CAP of the fresh ciliate isolates differ (P < 0.05).
Singleton was removed.