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. 2018 Jul 10;2018:6235417. doi: 10.1155/2018/6235417

Figure 8.

Figure 8

The influence of NAC pretreatment in resveratrol-induced ROS generation and cell proliferation. THJ-16T cells were pretreated with NAC (5 mM) or without NAC (5 mM) for 1 hour and then treated with or without 100 μM resveratrol for 48 h. (a) MTT assay performed on THJ-16T cells of different treatment. (b) Flow cytometer determination of intracellular ROS levels in THJ-16T cells of different treatment. (c) THJ-16T cells of different treatment were stained with DCFH-DA and MitoSOX. The statistical significance was set at ∗P < 0.05. N: normal culture; NAC: treatment with 5 mM NAC; R12/R48: treatment with 100 μM resveratrol for 12 h or 48 h; and NAC + R12/R48: pretreatment with 5 mM NAC for 1 h and subsequently exposed to 100 μM resveratrol for 12 h or 48 h.