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. 2018 Jul 31;9:293. doi: 10.3389/fgene.2018.00293

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Copyright © 2018 Zhang, Song, Ji, Feng, Yu, Nyachoti and Yang.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Zearalenone exposure increasing apoptosis and inducing the apoptosis-related gene mRNA abundance in cultured GCs. GCs were stained blue with Hoechst33342 Solution. TUNEL assay was performed using immunostaining. (A) Immunofluorescent staining of TUNEL and Hoechst33342 of GCs. Bar indicates 50 μm. (B) The percentages of TUNEL positive GCs. (C) The mRNA levels of BAX/BCL2 in cultured GCs exposed to ZEA, and the control determined by Q-PCR. The results are presented as mean ± SD. All experiments were repeated at least three times. ^∗P < 0.05; ^∗∗P < 0.01.