Figure 1.

Preincubation with CoQ₁₀H₂ prevented H₂O₂-induced senescence of HUVECs. (a) Representative images of SA-β-Gal-stained cells from each group (control, CoQ₁₀H₂, H₂O₂, and CoQ₁₀H₂ + H₂O₂). (b) Percentage of SA-β-Gal-positive cells (n = 4). (c) Expression levels of PAI-1 mRNA (n = 10). (d) Expression of genes involved in the senescence-associated secretory phenotype. Histograms show fold change in mRNA level relative to control cells (n = 6–9). ^∗ P < 0.05, ^∗∗ P < 0.01, and ^∗∗∗ P < 0.001; one-way ANOVA followed by Tukey's test.