Table 2.
Primary information of genotyped SNPs in Th17/IL23 pathway's genes.
| Gene SNPs |
Base change | MAF | Chromosome regions | Localization | Primers | Enzyme |
|---|---|---|---|---|---|---|
| IL23R rs1884444a |
G/T nonsens |
0.47 | Chr1 1p31.3 |
Exon2 | F:Fam✪ TGCTCTGTTTCCTTCCTTCC R1:CATCCCATTGAATAGTGACC R2:T4CATCCCATTGAATAGTGACA |
— |
| IL23R rs7517847 |
G/T | 0.36 | Intron6 | F:ATTTCTGGATGCCCTTTCCT R:ACATGAATTTGAGGGGCCTA |
BbvI | |
| IL23R rs11209026 |
A/G Arg-gln |
0.022 | Exon8 | F:TTAGACAACAGAGGAGACATTGGA R:CATGTAGTCTAAATCAGAAAACAGAAA |
Hpy188I | |
| IL23R rs10889677 |
A/C | 0.39 | 3′UTR | F:TGCTGGGCCATATGATAAGC R:TCCACCTTCGGGACCTTAAT |
MnII | |
| RORγt rs9645406a |
T/C | Chr1 1q21.3 |
Intron | F1:T4CCTCACAGCAAATCTTTTCTC F2:CCTCACAGCAAATCTTTTCTT R:NED✪AAAAACCGCTGTAGGGATCA |
— | |
| IL17A rs2275913 |
A/G | 0.29 | Chr6 6p12.2 |
5′UTR | F:ATTTCTGCCCTTCCCATTTT R:TTGTGCCTGCTATGAGATGG |
EarI |
| IL17A rs3748067 |
T/C | 0.077 | 3′UTR | R:GGGGCGAAAATGGTTACGAT F:AAGGCCCCTCAGAGATCAAC |
ApoI | |
| IL17F rs763780 |
C/T | 0.093 | Chr6 6p12.2 |
Exon3 | F:TGGGTAAGGAGTGGCATTTC R:CTGCATCAATGCTCAAGGAA |
NlaIII |
| IL17RA rs4819554 |
G/A | 0.233 | Chr22 22q11.1 |
5′UTR | F:TGAAATGTGTAATTCGCTGGC R:TGCTTTCCTTGGCTTTGCTT |
PvuII |
| TNFα rs1800629 |
A/G | 0.090 | Chr6 6p21.33 |
5′UTR | F:AGGCAATAGGTTTTGAGGGGCAT R:TCCTCCCTGCTCCGATTCC |
NcoI |
| STAT3 rs744166 |
C/T | 0.482 | Chr17 17q21.2 |
Intron | F:GCTGGAGTACAAACCCTGAA R:TGGTATTCAGATGGCGGTCA |
AluI |
aGenotyped using the AS-PCR method.