Figure 4.

AMPK inactivation restored inhibited adipocyte differentiation by hinokitiol in mesenchymal stem cells. MSCs in the presence of compound C or AICAR for 1 h were incubated with 10 μM of hinokitiol, following treatment with MDI. Western blot for LC3 and p62 proteins was analyzed from MSCs. β-Actin was used as loading control (a–d). The cells were immunostained with pAMPK antibody (red) and observed in fluorescent view (e). MSCs were incubated with AICAR at various concentrations (2.5, 5, 10, and 20 μM) following treatment with MDI, and Western blot for LC3 and p62 proteins was analyzed (f, g).