Fig. 2.
Sirt3-TG mice are protected against HFD-induced chronic liver injury. A–B. Liver sections with haematoxylin and eosin (H&E) staining. C–D. Hepatosteatosis was revealed by Oil Red O staining. E–F. Hepatic fibrosis was revealed by Sirius Red staining. G–L. Western blotting was performed to analyse the expression of proteins related to liver fibrosis. M–O. The levels of inflammatory factors in the blood were evaluated via ELISA. P–S. The liver tissues of WT mice and Sirt3-TG mice were isolated and western blotting was carried out to analyse the expression of pro-inflammation factors. The data represent the mean ± SEM (n = 4 mice per group). *P < 0.05.