Fig. 3.

Sirt3 alleviates high-fat-induced hepatocyte death. A. Primary hepatocytes were isolated from WT mice and Sirt3-TG mice. Then, PA was used in vitro to mimic the high-fat damage. MTT assay was used to analyse the hepatocyte viability in response to PA treatment. B–C. TUNEL staining for apoptosis detection. The green nucleus indicates an apoptotic cell. D–E. The ROS production in Sirt3-overexpressing cells with PA treatment was measured via flow cytometry. F–H. The levels of antioxidants, including SOD, GSH and GPx, were measured via ELISA. I. For analysis of mitochondrial function, cellular ATP production was evaluated via ELISA. The data represent the mean ± SEM (n = 4 mice per group). *P < 0.05.