Fig. 4.

Sirt3 sustains mitochondrial function and blocks mitochondrial apoptosis activation. A. The mitochondrial membrane potential was evaluated using TRME. The relative TMRE fluorescence was evaluated using spectrophotometer. B–E. After PA treatment, hepatocytes were collected, and the proteins were isolated. Then, western blotting was performed to analyse the expression of the mitochondrial respiratory complex. Tom20 was the loading control for the mitochondrial proteins. F–G. Immunofluorescence assay for cyt-c liberation. DAPI was used to tag the nucleus. The fusion of cyt-c and DAPI indicates the leakage of cyt-c from mitochondria into the cytoplasm/nucleus. H–M. The anti-apoptotic and pro-apoptotic protein expression was detected to quantify the mitochondrial apoptosis using western blotting analysis. The data represent the mean ± SEM (n = 4 mice per group). *P < 0.05.