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Hyperlipemia-inhibited mitophagy is re-activated by Sirt3 overexpression via Bnip3. A–G. After PA treatment, hepatocytes were collected, and the proteins were isolated. Then, western blotting was performed to analyse the proteins expression related to mitophagy. H–J. Immunofluorescence assay for mitophagy. The location of mitochondria and lysosomes indicates mitophagy. The number of mitophagy events was recorded. The data represent the mean ± SEM (n = 4 mice per group). *P < 0.05.
