Fig. 7.

Sirt3 modulates Bnip3 via the ERK-CREB signalling pathway. A–D. Proteins were isolated from PA-treated hepatocytes, and then, western blotting was carried out to analyse the expression of ERK phosphorylation, CREB phosphorylation and Bnip3. To inhibit the activity of ERK, PD98059 was applied to Sirt3-overexpressing cells. E–G. Immunofluorescence assay for ERK phosphorylation and Bnip3. PD98059 was used to block the ERK activation. H–I. The number of mitophagy events was monitored via immunofluorescence. The data represent the mean ± SEM (n = 4 mice per group). *P < 0.05.