Fig. 8.

The ERK-CREB signalling pathway is involved in PA-mediated hepatocyte apoptosis. A–B. TUNEL assay was used to observe the apoptotic index of hepatocytes with ERK inhibition using PD98059. The number of apoptotic cells was recorded. C–F. Western blotting for the inflammatory response via measuring the levels of inflammatory factors. G. ATP production in Sirt3-overexpressed cells with ERK inhibition. H–I. ROS production was measured via flow cytometry. The data represent the mean ± SEM (n = 4 mice per group). *P < 0.05.