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A
mRNA expression in FACS‐isolated Lin(Ter119/CD31/CD45)−Sca1+ progenitor cells from female mouse subcutaneous fat, differentiated in the presence of 1 μM cPGI2 or vehicle for the indicated time, as determined by expression profiling (n = 3, E‐MTAB‐3693). ****P = 3 × 10−6 (Day 2), ****P = 4 × 10−7 (Day 4), ****P = 1 × 10−6 (Day 6) in 2 × 2 ANOVA with Bonferroni's posttests (cPGI2 vs. vehicle).
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B
mRNA expression in MACS‐isolated Lin−Sca1+ progenitor cells from female mouse subcutaneous fat, differentiated in the presence of 100 nM Rosi or vehicle for the indicated time, as determined by qRT–PCR (n = 4). ****P = 1 × 10−10 (Days 1 and 2), **P = 0.001, in 2 × 2 ANOVA with Bonferroni's posttests (Rosi vs. vehicle).
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C
mRNA expression in female Lin−Sca1+ cells, differentiated in the presence of 100 nM Rosi or vehicle for 8 days, as determined by qRT–PCR (n = 3). t‐test Cited4
−/− vs. Cited4
+/+ (Rosi) *P = 0.013 (Ucp1), **P = 0.004 (Cpt1b), *P = 0.026 (Dio2).
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D–F
mRNA expression in primary SVF cells from human subcutaneous fat, differentiated in the presence of 100 nM Rosi (D) or vehicle (D–F), as determined by qRT–PCR at the indicated time points (n = 5 patients). ♀/♂ represents individual data. (D) ****P = 3 × 10−5 (Day 2), ****P = 3 × 10−6 (Day 6), ****P = 4 × 10−9 (Day 10), ****P = 3 × 10−7 (Day 14), in 2 × 2 ANOVA with Bonferroni's posttests (Rosi vs. vehicle). (E, F) Pearson correlation coefficient (r) and P‐value are shown.
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G
mRNA expression in primary SVF cells from human female subcutaneous fat transfected with the indicated siRNA prior to differentiation in the presence of 100 nM Rosi for 9 days, as determined by qRT–PCR (n = 3). ***P = 0.0002 (CITED4), **P = 0.002 (UCP1), *P = 0.02 (UCP1), *P = 0.035/0.026 (PPARG), ***P = 0.0006 (SLC2A4), **P = 0.002 (ADIPOQ) in one‐way ANOVA with Tukey's posttests (vs. siCtrl).
Data information: Data are presented as mean ± SEM except for (D) ♀/♂, (E and F) individual data.
