Figure 2. Cited4 deficiency in progenitors affects Ucp1 protein expression and β3‐adrenoreceptor mediated uncoupled respiration.

• A–C
  Quantitative fluorescence microscopy of LipidTOX‐ and DAPI‐stained female Cited4 ^F/F Lin⁻Sca1⁺ progenitor cells transfected with Cre or control mRNA prior to differentiation in the presence of 100 nM Rosi for 8 days (n = 5). **P = 0.002 in t‐test (Cre vs. Ctrl). Scale bar is 10 μm.
• D, E
  Ucp1 expression in female Cited4 ^F/F Lin⁻Sca1⁺ progenitor cells transfected with Cre or control mRNA prior to differentiation in the presence of 100 nM Rosi for 8 days, as determined by Western blot with VCP as loading control (n = 3). ***P = 0.0008 in t‐test (Cre vs. Ctrl).
• F
  Cellular respiration in female Lin⁻Sca1⁺ progenitor cells differentiated in the presence of 100 nM Rosi for 8 days. The extracellular oxygen consumption rate (OCR) was determined upon injection of the indicated substances and normalized to DNA content. CL: CL‐316243. Representative experiment (n = 9). *P = 0.041 (47 min), **P = 0.009 (55 min), **P = 0.003 (62 min) in 2 × 2 ANOVA with Holm–Sidak posttests (Cited4 ^−/− vs. Cited4 ^+/+).
• G
  Normalized CL‐stimulated uncoupled respiration of cells in (F). Values represent the means of the three time points after CL injection after subtraction of the means of the oligomycin time points (n = 9). *P = 0.027 in t‐test (Cited4 ^−/− vs. Cited4 ^+/+).

Data information: Data are presented as mean ± SEM.Source data are available online for this figure.