Figure 3. Cited4‐ and Rosi/PPARg‐dependent transcriptional programs overlap in differentiating adipocyte progenitors.

• A
  MA‐plot of RNA expression profiles from female Lin⁻Sca1⁺ progenitors 2 days after induction of differentiation with 100 nM Rosi, displaying the log₂‐ratio of Cited4 ^−/− to Cited4 ^+/+ intensities against the average log₂‐intensities for all microarray probe sets (n = 3). t‐test with Welch's correction on Cited4 ^−/− vs. Cited4 ^+/+ (Rosi).
• B
  Comparison of the lists of genes significantly changed by Rosi in wild‐type cells (Cited4 ^+/+: Rosi vs. Ctrl, P < 0.01) or by Cited4‐knockout under Rosi treatment (Rosi: Cited4 ^−/− vs. Cited4 ^+/+, P < 0.01) in expression profiles from female Lin⁻Sca1⁺ progenitors 2 days after induction of differentiation with 100 nM Rosi or vehicle (n = 3, t‐test with Welch's correction).
• C
  Comparison of the lists of gene sets significantly enriched by Rosi in wild‐type cells (Cited4 ^+/+: Rosi vs. Ctrl, false discovery rate (FDR) <0.1) or by Cited4‐knockout under Rosi treatment (Rosi: Cited4 ^−/− vs. Cited4 ^+/+, FDR < 0.1) in GSEA (KEGG) (n = 3).
• D–G
  Enrichment plots from GSEA with the KEGG (D–F) or TFT (G) gene set collection (Cited4 ^−/− vs. Cited4 ^+/+), performed on RNA expression profiles 2 days after induction of differentiation with 100 nM Rosi (n = 3). Vertical bars represent the individual genes of the gene set.
• H
  Comparison of the lists of gene sets affected by Cited4‐knockout under Rosi or under vehicle treatment (Cited4 ^−/− vs. Cited4 ^+/+, FDR < 0.1) in GSEA as in (C).

Data information: Data points in (A) and vertical bars (D–G) represent means for individual genes.