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. 2018 Jun 21;10(8):e8304. doi: 10.15252/emmm.201708304

Figure 1. LIMD1 expression is regulated by hypoxia.

Figure 1

The indicated panel of cell lines was exposed to either normoxia (20% O2) or hypoxia (1% O2) for up to 48 h prior to RNA and protein extraction.
  • A, B
    (A) LIMD1 mRNA and (B) protein levels were increased following hypoxic exposure.
  • C
    Densitometric analysis of (B).
  • D
    The LIMD1 promoter contains a hypoxic response element responsible for HIF binding and transcriptional activation of LIMD1. Three predicted HRE elements were individually deleted within the context of the wild‐type LIMD1 promoter‐driven Renilla luciferase.
  • E
    Reporter constructs in (D) were expressed in U2OS cells and exposed to hypoxia for the indicated time‐points. Luciferase activity was then assayed and normalised to firefly control. Data are displayed normalised to the normoxic value for each construct. Deletion of the third HRE present within the LIMD1 promoter (ΔHRE3) inhibited hypoxic induction of LIMD1 transcription.
  • F, G
    (F) Sequence alignment and (G) sequence logo of LIMD1 promoters from the indicated species demonstrate that the HRE3 consensus sequence is highly conserved.
Data information: Unless otherwise stated, data shown are mean ± SEM, n = 3, *P < 0.05, **P < 0.01, ***P < 0.001, according to the Student's t‐test (A) or Holm–Šidák post hoc tests, comparing time‐points within each cell line (A and C) or comparing the VO group to every other genotype within each time‐point (E), following significant main effects/interactions of a mixed‐model ANOVA. See Appendix Table S1 for a summary of statistical analysis.Source data are available online for this figure.