Figure 4.

a. Cisplatin sensitivity testing of A2780 and A2780cis cell lines. Cells were incubated as five replicates for 3 days with increased concentrations of cisplatin as indicated and cell-viability was measured by CTG assay. EC50 was calculated using Graph Prism software and shown as approximate values.

b. ROR2 mRNA expression in platinum sensitive and resistant cell lines A2780/A2780cis (P = .0046). The graphs show mean ± SEM.

c. GREB1 mRNA expression in platinum sensitive and resistant cell lines A2780/A2780cis (P = .0012. The graphs show mean ± SEM.

d. Protein expression levels for ROR2, Wnt5a, NF-kB and STAT3 in the cytoplasmic and nuclear cell lysates of A2780 and A2780cis cell lines. Protein quantification was done using Oddyssey Licor software and normalized against the loading control.