Table 3.

β-Lactamases and other β-lactam resistance proteins encoded in the Cedecea neteri SSMD04 genome.

Locus tags∗	Annotated gene products	Molecular class†	Coding genes	Sequence signatures (amino acid positions)
JT31_00700	Metallo-β-lactamase	B	bla MBL	T-H-x-H-x-D-H-x-G-G (128–137)‡
JT31_03975	Metallo-β-lactamase	B	—	S-x-x-K (72–75)§
JT31_07450	Muropeptide transporter, β-lactamase induction signal transducer	NA	ampG	Major Facilitator Superfamily (MFS) domains (17–366, 319–481)
JT31_10465	LysR family transcriptional regulator	NA	ampR ¶	HTH domain (8–67); LysR substrate-binding domain (91–289)
JT31_10470	β-Lactamase	C	ampC ¶	S-x-S-K (85–88)
				Y-A-N (171–173)
				S-D-N-K (308–311)
				K-T-G (336–338)
JT31_16535	Metallo-β-lactamase	B	bla MBL	H-x-H-x-D-H (156–161)
JT31_22070	Metallo-β-lactamase	B	bla MBL	H-x-H-x-D-H (131–136)
JT31_22350	β-Lactamase	A/D	—	S-x-x-K (144–147)

^∗KEGG Genome database, Cedecea neteri strain SSMD04 (http://www.genome.jp/kegg-bin/show_organism?org=cnt). ^†The molecular classification of β-lactamases is based on the primary amino acid sequence of these enzymes (also known as the Ambler classification scheme). For a review, see [19]. NA, not applicable. ^‡Sequence signature contains the core H-X-H-X-D motif characteristic of class B β-lactamases, but also contains additional residues reported in [20] that constitute an expanded motif specific to metallo-β-lactamases. ^§Only one conserved sequence element was identified in the deduced amino acid sequence of ORF JT31_03975. Motif S-X-X-K is characteristic of class A and D β-lactamases. The absence of the conserved zinc ion interaction domain (H-X-H-X-D) suggests that this ORF may encode a novel metallo-β-lactamase or a variant class A or D β-lactamase. ^¶Proposed gene name for this C. neteri SSMD04 ORF based on sequence analysis (this study).